Authored By: B. V Waghmare

Monday, April 7, 2008

Evolution of the liposome as a source for targeted drug delivery system for anti AIDS drugs ,and as a GEN carrier

Liopsomes are Great source for reaserch on drugs on HIV,
liposomes can be designed and taged with CD4 counter receptors and , a gen can be encapsulated in it, for delivery in infected cell precisely.

Agent which can inhibit elongation and Transcription of HIV PROVIRUS DNA, can be delivered by using Liposomes.

The drug delivery system (DDS) is attractive as a therapeutic method. Liposomes are of particular interest as a DDS because they can reduce drug toxicity, and offer promise as gene carriers. An evolution has occurred in the construction of liposomes in the effort to develop efficient vectors for in vivo use. To avoid uptake by the reticuloendothelial system (RES); Lipid components have been optimized. To enhance tissue targeting, liposome surface has been modified with antibodies or ligands recognized by specific cell types. To enhance the efficiency of gene delivery by the introduction of molecules directly into cells, virosomes have been developed by combining liposomes with fusiogenic viral envelope proteins. Liposomes are now being used in the treatment of intractable human diseases such as cancer and monogenic disorders. In the future, many medical procedures will be performed using liposomes.

The proposed reversed-phase high-performance liquid chromatography method with ultraviolet detection provides a simple and rapid procedure to separate and quantitate lipids from cationic liposomes used in gene transfer. We describe experimental conditions which do not require lipid extraction from liposomes prior to sample analysis. Evaluation of the method reported here showed suitable lipid separation capacity and quantitation accuracy from cationic liposomes composed of either the pentammonio lipid pcTG90 and dioleoyl phosphatidylethanolamine, or 1,2-dioleoyl-3-trimethylamonium propane and cholesterol. Detection limits were in the range of 0.5–1 μg depending on the lipid. This quantitative method has proven useful in lipoplex formulation processing development and its application may be extended to a wide range of lipid-based gene and drug delivery systems.
Source :

Liposomal Drug delivery system and HIV

Delivery of anti-HIV-1 Rev-binding aptamer and ribozyme DNAs through liposomal drug delivery system

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Last updated: 07-Apr-2008

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